Laser Capture Microscopy

Zeiss PALM Laser Microbeam Microscope (located in HSRB)

Advanced Technology - Uses Laser Microdissection and Pressure Catapulting (LMPC) technology to obtain contamination-free samples from various sources.

Sources

• Histological specimens
• Living cells and cell cultures
• Plant material
• Chromosome spreads
• Forensic preps

Preparation

• Cryofixation or FFPE material
• Living or fixed
• Stained or unstained
• For fluorescence and transmitted light

Capabilities

• Energy can be narrowed to a focal point of less than 1 μm
• Microdissection and micromanipulation at a subcellular level
• Isolate single nuclei, filaments, chromosomes, or even chromosomal parts
• Isolate a single live cell out of a heterogeneous cell culture without affecting viability
• Adhesive cap capture tubes enable liquid free sampling
• Membrane slides allow captured specimens to maintain their morphology

Subsequent Analyses

• Analyze DNA, RNA and proteins
• Hybridize to Microarrays, PCR, RT-PCR, 2-D PAGE, immunoblot
• Recultivate live cells

Microscope

• Axio-Observer—An inverted research microscope with a newly designed fluorescence beam path. AxiCam MRc and MRm allow both brightfield and fluorescence high-resolution Digital Imaging.
• AxioVision System Software—Additional functionalities, featuring Extended Focus and Multichannel Fluorescence.