Facility Overview

Services and Support

Imaging Facility staff provide technical support for preparation of specimens used in confocal, laser capture and electron microscopy, and histology on a fee basis. Preparation of fixative and buffer solutions can be provided at the investigator's request. Immuno-labeling using fluorescence (light level), ABC-peroxidase (light and ultra-structural levels) and colloidal gold (ultra-structural level) is also available. Investigators are required to provide their own primary antibodies or detection antibodies that are not stocked by the facility. Investigators may choose the level of technical support based upon their individual needs. Support may range from instrumentation training (allowing independent use by the investigator) to complete processing of samples and final imaging by the facility staff. Electron Imaging Fees (PDF, 8pgs).

Available Equipment

Confocal Microscopy

• Leica SP Inverted Confocal Microscope
• Leica SP5 AOBS Inverted Confocal Microscope with Environmental Chamber

• Leica TCS SP5 Inverted Confocal with Multiphoton Laser

Laser Capture Microscopy

• Zeiss PALM MicroBeam Laser Capture Microscope

Electron Microscopy

• Philips BioTwin 12 Transmission Electron Microscope with STEM, EDAX and Gatan 1000 ES Digital Camera
• Hitachi 7100 Electron Microscope
• Reichert Ultracut E Microtomes (2)
• Leica UC6 Ultramicrotome
• Leica EM KMR2 Knifebreaker

Histology

• Leica VT 1000 S Vibratome
• Shandon Cryotome FSE
• Hacker Cryostat
• Shannon Finesse Paraffin Microtomes (2)
• LX120 Tissue Processor
• Leica Histo Embedder
• Thermo Shannon Slide Stainer
• H/I-76 Knife Sharpener
• Shandon Excelsior Tissue Processor